Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses

Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses

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We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC.Once established, ATCi-MF1 cells present a normal Console Up karyotype, are Sendai virus-free and express pluripotency associated markers.Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts.Pluripotency was tested with the in vivo teratoma formation assay.

ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells Headwear in pre-implantational embryos.